Objective the purpose of this experiment is to identify an unknown proprietary drug using thinlayer chromatography. Analgesics act in various ways on the peripheral and central pain pathways and are regarded as one of the. The possible unknowns and their analgesic ingredients will be anacin aspirin, caffeine, excedrin acetaminophen, caffeine, aspirin, motrin. Thin layer chromatography of the unknown analgesic free. To assess the purity of the colored organic compounds provided to you, using tlc. From the array of components you discover by tlc, you will be able to identify the otc drug name from table 1. In this experiment, thin layer chromatography tlc will be used to identify the active ingredients in some of these products. Thin layer chromatography tlc analysis of analgesic drugs. Introduction thin layer chromatography also known as tlc is the physical separation of a mixture into its individual components by distributing the components between a stationary phase the porous tlc plate and a mobile phase the solvent that moves through the stationary phase and carries the material that needs. The use of thin layer chromatography in the examination of. In this experiment, you will use tlc to identify unknown analgesic painkillers using the table of analgesics and their components in the experimental section of this experiment.
Jpc journal of planar chromatography modern tlc 2019, 32 6, 501504. There are many forms of chromatography, but one thing that remains constant throughout all of the types of chromatography is. Aspirin, acetaminophen, and caffeine separation from excedrin via column chromatography. Identification of analgesic drugs tlc cerritos college.
Experiment 12 tlc analysis of analgesic drugs free download as word doc. The unknowns behavior in thinlayer chromatography will be compared with that of its possible component analgesics. Be careful with the spotting capillaries, they can be sharp be careful when making the spotting capillaries, glass heated in a flame is hot. Tlc can be used to help determine the number of components in a mixture, the identity of compounds, and the purity of a compound.
Pdf chromatography analyzing analgesics by tlc and. Identification of overthecounter analgesics thinlayer chromatography tlc is a fast and simple method that can be used to identify organic molecules, verify the number of components in a mixture, or to monitor the. Thin layer chromatography in drug analysis crc press book. Filling the need for an uptodate, complete reference, thin layer chromatography in drug analysis covers the most important methods in pharmaceutical applications of tlc, namely, analysis of bulk drug material and pharmaceutical formulations, degradation studies, analysis of biological samples, optimization of the separation of drug classes. It may be performed on the analytical scale as a means of monitoring the progress of a reaction, or on the preparative scale to purify small amounts of a compound. In this experiment, a thinlayer chromatography tlc was used to determine the composition of various overthecounter analgesics. In this lab the objective was to determine the active ingredients. Tlc analysis of analgesics chem 21l unc charlotte studocu. Learn about how chemicals can be separated based on polarity through thin layer chromatography tlc. You will be given five known reference substances, an unknown. Thin layer chromotography tlc can be used to determine the purity of compound, to analyze the composition of a mixture are differentiated by exposing to two competing phases, the stationary phase is a polar adsorbent such as silica gel or alumina, which has been coated on plastic plate. Acetaminophen, acetylsalicylic acid, caffeine, and ibuprofen. Thin layer chromatography introduction thin layer chromatography or tlc is a technique used as a separation and identification technique. May 03, 20 antipyretic, antiinflammatory and analgesic drugs.
Students extract, isolate, and perform thin layer chromatography on aspirin, acetominophen, or ibuprofen. To determine the identities of the analgesic components present in an unknown mixture by comparison with known analgesic samples using tlc. The purpose of this experiment is to focus on the ingredients that the analgesic drugs may contain. Ideal for the first week of an organic chemistry sequence to get students familiar with their surroundings. Oct 19, 20 chromatographic are used extensively in organic chemistry laboratories for routines analysis. E29 preparation of aspirin acetylsalicylic acid and thin. If you use any reference, please, write the complete. Thin layer chromatography, or tlc, is a method for analyzing mixtures by separating the compounds in the mixture. All of them should be written in paragraphs and the method should not be copied exactely from the instruction. Identification of overthecounter analgesics thinlayer chromatography tlc is a fast and simple method that can be used to identify organic molecules, verify the number of components in a mixture, or to monitor the progress of a reaction. Thin layer chromatography of the unknown analgesic jessica bajao, phoebe abalos, kevin antiga, carmelus aseneta 3biology 2 college of science, university of santo tomas, manila, philippines abstract the group used five different analgesics in this experiment.
Salicylic acid is the substance that has analgesic, antiinflammatory, and antipyretic properties. Essentially, tlc has many purposes within the setting of lab. Thin layer chromatography tlc analysis of analgesic drugs reading experiment 12 in pavia 5th edition pp 9195. Separation of the components of an analgesic tablet ii. Learning objectives understand the basic principles of polarity of organic compounds. In this lab, you will perform thin layer chromatography tlc to identify an unknown drug and then use column chromatography to separate out the components of. Elgebalyc aanalytical chemistry department, faculty of pharmacy, benisuef university,62111 benisuef, egypt. Design of a drug discovery course for nonscience majors. Spot one plate with your 4 known standards acetaminophen, aspirin, caffeine, and ibuprofen and the other plate with the 5 unknown. Lab report on tlc analisys of analgestic drugs essay examples 1266 words 6 pages tlc analysis of analgesic drugs introduction in this experiment, thinlayer chromatography tlc was used to determine the composition of various overthecounter otc analgesics. Chromatographic are used extensively in organic chemistry laboratories for routines analysis. Tlc analysis of analgesic drugs introduction in this experiment, thinlayer chromatography tlc was used to determine the composition of various overthecounter otc analgesics. Aspirin, acetaminophen, and caffeine separation from. Thin layer chromatography lab report freebooksummary.
Thin layer chromotography of analgesic drugs objectives. Tlc chromatographicdensitometric assay of ibuprofen and its. Simultaneous densitometric determination of aspirin and omeprazole by highperformanee thinlayer chromatography. Precut plastic backed or metal backed tlc plates, tlc capillaries, uv lamp aim the goal of your experiment is two fold. Tlc analysis of analgesic drugslab report 1 free download as word doc. Pdf in organic chemistry, chromatography refers to the separation of a mixture or ions based. Hi,this experiment is abouttlc analysis of analgesic drugsand i would like from you to write a report. Thin layer chromatography rev 22017 thin layer chromatography tlc is a method for analyzing mixtures by separating the compounds in the mixture based on polarity. In combination with nonsteroidal antiinflammatory drugs nsaids and opioid analgesics, paracetamol is used also in the management of severe pain such as. Thinlayer chromatography of analgesics introduction in this experiment, you will use thin. An analgesic or painkiller is any member of the group of drugs used to achieve analgesia, relief from pain.
Analgesics are any compounddrug that provides relief from pain. January 24, 20 tlc analysis of analgesic drugs introduction. Thin layer chromatography tlc is a method for analyzing mixtures by separating the. Hplc method for the analysis of paracetamol, ca eine and. Individual variability in pain perception and differences in the efficacy of analgesic drugs are complex phenomena and are partly genetically predetermined. Your drug may contain from one to three of these substances. Apr 11, 20 thin layer chromatography introduction thin layer chromatography or tlc is a technique used as a separation and identification technique. Draw a light pencil line about 1 cm from the end of each chromatographic plate. Read all of the required reading at the beginning of the experiment, especially analgesics p. They are distinct from anesthetics, which temporarily affect, and in some instances completely eliminate, sensation. Tlc analysis of analgesic drugs flashcards quizlet. Be able to deduce the relative polarity of organic compounds by comparing the functional groups. Pdf thin layer chromotography of analgesic drugs idayu.
Analysis of drugs by thin layer chromatography experimental techniques required chromatography thinlayer t other documents. An introductory laboratory on the identification of analgesics in an unknown sample. Method iv is tlc qr code for mobile users different spectrophotometric and tlc densitometric methods for determination of two analgesic drugs nada s. Analgesic drugs act in various ways on the peripheral and central nervous systems. E29 preparation of aspirin acetylsalicylic acid and thinlayer chromatography of analgesic drugs the task to prepare a pharmaceutical compound and test its purity.
As the reaction proceeds, the solid salicylic acid disappears and the acetylsalicylic acid product. Overthecounter otc analgesics typically contain one or more of the following active. Pdf different spectrophotometric and tlcdensitometric. Thin layer chromatography analysis of analgesic drugs essay sample. Essay about tlc analysis of analgesic drugs 625 words cram. A method for the thinlayer chromatography of analgesic. Method iv is tlc qr code for mobile users different spectrophotometric and tlcdensitometric methods for determination of two analgesic drugs nada s. May 11, 2014 hi,this experiment is abouttlc analysis of analgesic drugsand i would like from you to write a report. Almost all mixtures of solvents can be used as the mobile phase. Excedrin is an overthecounter pain reliever that contains three analgesic components. Essay lab report on tlc analisys of analgestic drugs. These include highpressure liquid chromatography hplc, gas chromatography gc, column chromatography, paper chromatography, and the technique you will be using in this experiment tlc. Six analgesics were spotted on the tlc plate including the unknown. Thin layer chromatography analysis of analgesic drugs essay.
Tlc analysis of analgesic drugs aim of the experiment determine the composition of a series of. Different spectrophotometric and tlcdensitometric methods for determination of two analgesic drugs article pdf available april 2014 with 55 reads how we measure reads. Essay about tlc analysis of analgesic drugs 7 words cram. Solved experiment 3 tlc analysis of analgesic drugs. Thinlayer chromatography mohrig, 2nd edition, technique 15, pp. There are many forms of chromatography, but one thing that remains constant throughout all of the types of chromatography is that there is a stationary phase and a mobile phase.
Nonnarcotic or nonopioid nsaid,s and aspirin like analgesics. Overthecounter otc analgesics typically contain one or more of the following active ingredients. The combination of paracetamol with dipyrone is used as an antipyretic, analgesic and antiin. Lab report on tlc analisys of analgestic drugs essay examples.
Tlc key thinlayer chromatography lab complete answer key. A few common analgesic drugs will be analyzed and an unknown analgesic drug will be identified via the thin layer chromatography technique. Application of tlc densitometry method for simultaneous. All of them should be written in paragraphs and the. Experimental procedure, report template introduction in this experiment, thin layer chromatography tlc will be used to examine the composition of various analgesic painrelieving drugs by comparison with the. Numerous methods have been reported for the analysis of paracetamol and its combinations in pharmaceuticals or in biological. In this experiment, thin layer chromatography tlc will be used to examine the composition of various analgesic painrelieving drugs by comparison with the. Have you taken the safety quiz on the chemsafety quiz sakai site.
Experiment 12 tlc analysis of analgesic drugs thin layer. In this experiment, you will use tlc to identify the components of the analgesic drug that your instructor assigns. Different spectrophotometric and tlcdensitometric methods. Tlc analysis of analgesic drugslab report 1 thin layer. In thinlayer chromatography, the stationary phase is a polar absorbent, usually finely ground alumina or silica particles. Caffeine has been an additive in analgesics for many years. The skills by the end of the experiment you should be able to. A benefitrisk assessment of caffeine as an analgesic adjuvant. Tlc key thinlayer chromatography lab complete answer. Transfer the crushed tablet to a small test tube and add 1 ml of ethanol. Thinlayer chromatography analysis of analgesics adam cap. Analyzing analgesics by thin layer chromatography tlc. This absorbent is coated on a glass slide or plastic sheet creating a thin layer of the particular stationary phase. Please turn in the answers to these questions when you arrive to your first lab.
Chromatography introduction column chromatography will be used to separate the components of extrastrength excedrin, or a generic of the same composition, a combination pain reliever that contains three active ingredients. The report should be up to 800 words and it should include. The purpose of this lab was to determine the composition of four different overthecounter drugs anacin, bufferin, excedrin, and tylenol. However, the analgesic adjuvant effects of caffeine have not been seriously investigated since a pooled analysis conducted in 1984 showed that caffeine reduces the amount of paracetamol acetaminophen necessary for the same effect by approximately 40%. Visualization and analysis when the tlc plate is dry, observe the spots under shortwavelength 254 nm ultraviolet light, outline them with a. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Thin layer chromotography tlc can be used to determine the purity of compound, to analyze the composition of a mixture are differentiated by exposing to two.
992 551 975 1544 604 1182 158 1356 973 178 51 264 1013 948 33 183 642 827 1223 531 1545 826 494 983 1079 1189 1464 141 1027 129 1366 1055 51 10 1083 303 230 1426 1437 1078 536